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streptavidin-coated glass microscope slides greiner bio-one 439003-25  (Greiner Bio)


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    Structured Review

    Greiner Bio streptavidin-coated glass microscope slides greiner bio-one 439003-25
    Materials
    Streptavidin Coated Glass Microscope Slides Greiner Bio One 439003 25, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glass+microscope+slides+greiner+bio+one/streptavidin+coated+slide+glass+plate/pmc05613814-2-0-5
    Average 90 stars, based on 1 article reviews
    streptavidin-coated glass microscope slides greiner bio-one 439003-25 - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Analysis of Histone Antibody Specificity with Peptide Microarrays"

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays

    Journal: Journal of visualized experiments : JoVE

    doi: 10.3791/55912

    Materials
    Figure Legend Snippet: Materials

    Techniques Used: Microscopy, Microarray, Blocking Assay, Plasmid Preparation, Hybridization, Peptide Microarray, Modification, High Throughput Screening Assay

    Related Articles

    Microscopy:

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays
    Article Snippet: .. Compatibility with the other arrayers can be configured upon request. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide. .. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide.

    Microarray:

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays
    Article Snippet: .. Compatibility with the other arrayers can be configured upon request. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide. .. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide.

    Blocking Assay:

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays
    Article Snippet: .. Compatibility with the other arrayers can be configured upon request. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide. .. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide.

    Hybridization:

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays
    Article Snippet: .. Compatibility with the other arrayers can be configured upon request. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide. .. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide.

    Peptide Microarray:

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays
    Article Snippet: .. Compatibility with the other arrayers can be configured upon request. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide. .. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide.

    Modification:

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays
    Article Snippet: .. Compatibility with the other arrayers can be configured upon request. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide. .. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide.

    High Throughput Screening Assay:

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays
    Article Snippet: .. Compatibility with the other arrayers can be configured upon request. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide. .. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide.



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    Image Search Results


    Materials

    Journal: Journal of visualized experiments : JoVE

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays

    doi: 10.3791/55912

    Figure Lengend Snippet: Materials

    Article Snippet: Streptavidin-coated glass microscope slides , Greiner Bio-one , 439003-25 , .

    Techniques: Microscopy, Microarray, Blocking Assay, Plasmid Preparation, Hybridization, Peptide Microarray, Modification, High Throughput Screening Assay

    Materials

    Journal: Journal of visualized experiments : JoVE

    Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays

    doi: 10.3791/55912

    Figure Lengend Snippet: Materials

    Article Snippet: Compatibility with the other arrayers can be configured upon request. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide.

    Techniques: Microscopy, Microarray, Blocking Assay, Plasmid Preparation, Hybridization, Peptide Microarray, Modification, High Throughput Screening Assay